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Schaedler Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes
Introduction of Schaedler Agar Schaedler Agar is recommended for the enumeration of various aerobic and anaerobic bacteria present in the gastrointestinal tract. Bacteroides fragilis grew on Schaedler Agar after 48 hours of anaerobic incubation as shown above picture. Principle of Schaedler Agar Tryptone, proteose peptone, Soya peptone, Yeast extract, and L-cystine supply nitrogenous growth factors, vitamins, and other essential growth nutrients. Dextrose/glucose serves as an energy source. Hemin and sheep blood enhances the growth of fastidious microbes and...
DHL Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes
Introduction of DHL Agar DHL Agar is the short name for Deoxycholate Hydrogen Sulfide Lactose Agar. It is a modification of Deoxycholate Agar and it was proposed by Sakazaki et. al. DHL agar supplies contain a rich nitrogen base to permit the growth of fastidious strains of Salmonella and Shigella species. The colonies formed are considerably larger than those found on similar selective media (MacConkey agar, S-S agar, etc.). Principle of DHL Agar Enzymatic Digest of...
Hektoen Enteric Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes
Introduction of Hektoen Enteric Agar Hektoen enteric agar was developed in 1968 by Sylvia King and William I. Metzger in an effort to increase the recovery of Salmonella and Shigella species over the previously formulated Salmonella-Shigella (SS) Agar. This is also known as HEK and HEA. This medium is particularly useful in the isolation of Shigella species. They formulated HE Agar medium while working at the Hektoen Institute in Chicago and became able to increase the...