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Virus Cultivation in the Cell lines: Introduction, Principle, Procedure, Result- Interpretation, and Keynotes
Introduction of Virus Cultivation in the Cell lines Viruses are intercellular pathogens that are dependent on host cell machinery for their multiplication and growth. Nowadays monolayer cell cultures are mostly used in diagnostic and research work in viral diseases. Cell culture is the most widely used system for the cultivation of viruses. It is a more convenient method compared to other methods like egg inoculation and animal inoculation. Candling of an egg is done prior to specimen...
Dubos Oleic Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes
Introduction of Dubos Oleic Agar Dubos Oleic Agar is commonly used for isolation and antimicrobial susceptibility testing (AST) of Mycobacterium tuberculosis . M. tuberculosis, the etiological agent of tuberculosis. The organism may present in airborne particles(droplet nuclei)that are generated when patients with pulmonary tuberculosis cough. The disease can happen when a susceptible person inhales the droplet nuclei containing M. tuberculosis. It is one of the common agar-based media for the isolation of mycobacteria. Principle of Dubos Oleic Agar...
Agarose Gel Electrophoresis: Introduction, Principle, Requirements, Test Procedure, and Applications
Introduction of Agarose Gel Electrophoresis Agarose gel electrophoresis is a method of gel electrophoresis. It is widely applied in various fields like biochemistry, molecular biology, genetics, clinical chemistry, etc. to separate a mixed population of macromolecules such as DNA, RNA, or proteins in a matrix of agarose. Agarose is a natural linear polymer and it is extracted from seaweed that forms a gel matrix by hydrogen bonding when heated in a buffer and allowed to cool. They are the most popular solid...