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Neomycin Blood Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes

Neomycin Blood Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes Introduction of Neomycin Blood Agar Neomycin Blood Agar is a selective medium used for the isolation of group A ( Streptococcus pyogenes) and B streptococci (Streptococcus agalactiae). Composition of Neomycin Blood Agar Typical Formula   gm/liter (BBL) Pancreatic Digest of Casein: 14.5 Papaic Digest of Soybean Meal: 5.0 Sodium Chloride: 5.0 Growth Factors: 1.5 Neomycin: 0.03 Sheep Blood defibrinated: 50 ml Agar: 14.0 g Principle of Neomycin Blood Agar The pancreatic Digest of Casein incorporation of medium...

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Yersinia Selective Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes

Yersinia Selective Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes Introduction of Yersinia Selective Agar Yersinia Selective Agar is recommended for the selective isolation and enumeration of Yersinia enterocolitica from clinical specimens (feces) as well as non-clinical sample like food samples, feces, etc. Image source:https://www.fishersci.com/ Principle of Yersinia Selective Agar The mannitol of the medium helps to differentiate between mannitol fermenting and non-fermenting bacteria. Microbes that ferment the sugar mannitol acidify the medium. It causes a localized drop in pH around the colonies.  The colonies take...

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Pseudomonas agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes

Pseudomonas agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes Introduction of Pseudomonas agar Pseudomonas agar is clear from its name and thus it is recommended selective isolation of Pseudomonas species. Principle of Pseudomonas agar The incorporation of tryptone and gelatin peptone provides nitrogenous and carbonaceous compounds, long chain amino acids, and other essential growth nutrients for the organisms. Magnesium chloride and potassium sulphate of medium enhance pigment production. Cetrimide and Sodium nalidixate used as a supplement makes  the medium specific for isolation of Pseudomonas from clinical specimens....

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