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Introduction of Tuberculin Skin Test Tuberculin skin test (TST) is also familiar with tuberculin test or tuberculosis skin test or Mendel–Mantoux test or tuberculin sensitivity test or purified protein derivative (PPD) tests. It is a tool for screening for tuberculosis and for tuberculosis diagnosis. The standard recommended tuberculin test is the Mantoux test and it determines if someone has developed an immune response to the bacterium that causes tuberculosis i.e. Mycobacterium tuberculosis. PPD uses in this test is the extract...

Introduction of D-Zone Test Putting clindamycin disk 15 mm away from the edge of an erythromycin disk is on an MHA plate incubating of organisms after 18–24 hours if shows flattening of the clindamycin zone of inhibition adjacent to the erythromycin disk appears as letter D zone and thus the test is called D-Zone test. It is applied for the detection of isolates whether inducible macrolide, lincosamide, streptogramin B resistance (MLSBi), or constitutive macrolide, lincosamide, streptogramin...

Introduction of Mice Footpad Injection Mice footpad injection is an important route of inoculation in mice models of disease and immunology.  Mice Footpad Injection or inoculation is a common experiment in Leprosy. As M. leprae cannot be cultured in-vitro but does grow on the footpads of mice, a conventional mouse colony was introduced to perform culture and sensitivity testing of M. leprae. In 1982 a nude mice colony was introduced to facilitate new drug trials for leprosy. Swiss Albino mice are the...