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Introduction Cryptococcal meningitis occurs in immunodeficient patients and when meningitis is clinically suspected, for example, patients with HIV disease, or when yeast cells with lymphocyte are detected when performing C.S.F. cell count or examine Gram smear, examine India ink preparation for encapsulated yeasts. India ink is used as a negative stain in negative staining that uses ion negative staining method permits visualization of the usually transparent and unstainable capsules of various micro- microorganisms like Cryptococcus neoformans (most commonly), Klebsiella...

 Introduction of Klebsiella pneumoniae Klebsiella pneumoniae scientific classification is as follows Domain: Bacteria Phylum: Proteobacteria Class: Gammaproteobacteria Order: Enterobacterales Family: Enterobacteriaceae Genus: Klebsiella Species:  pneumoniae Subspecies of Klebsiella pneumoniae are- Klebsiella pneumoniae subspecies ozaenae Klebsiella pneumoniae subspecies  pneumoniae Klebsiella pneumoniae subspecies rhinoscleromatis Klebsiella is from the surname of  German-Swiss microbiologist Edwin Klebs (1834–1913). Klebsiella is a genus of Gram-negative, oxidase-negative, fimbriated, non -motile, non-sporing rod-shaped bacteria with a prominent polysaccharide-based capsule and having size o,f   0.5...

 Code of practice to work in Microbiology Laboratory (Laboratory guidelines) The following laboratory guidelines should be followed to work in a Microbiology laboratory and they are- Mouth pipetting is prohibited.  Eating, drinking, smoking, storing food, and applying cosmetics are not permitted in the laboratory area. The laboratory must be neat and clean and free of materials not pertinent to the work.  Working surfaces are decontaminated at least once a day and after each spill of viable...