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Lactophenol cotton blue (LPCB) wet mount is not only used in mycology but also in parasitology laboratory to identify filamentous fungi and parasitic structures respectively
Lactophenol Cotton Blue (LPCB) wet mount Introduction LPCB is a combination of fixative, staining, and clearing agent. Its contents functions are as follows- Lactic acid: It helps in preserving the morphology of the fungal elements. Phenol: It acts as a disinfectant Cotton blue: It stains the fungal elements as well as intestinal parasitic (cyst, ova, and oocyst) and nonparasitic structures(vegetable cells, mucus, muscle fibers, and other artifacts) and Glycerol: It is a hygroscopic agent that prevents...
Anaerobic Infection: Importance, Indications, List of Anaerobic Bacteria
Anaerobic infection As you know, isolating the causative agent of anaerobic infection is really cumbersome. It means that leave the practice which is not a solution. This is not my personal opinion but according to researchers' data; "many reports associate 50-60% of important infections with anaerobic bacteria." This is the reason, we concern with anaerobic infections. The possibility of anaerobic infection indicated by the following clues and they are- The foul odor of the specimen site...
Cryptococcosis: Introduction, Pathogenesis, Laboratory Diagnosis, Treatment, Prevention and Control
Introduction of Cryptococcosis Cryptococcosis is an acute, subacute, or chronic pulmonary meningeal mycosis caused by Cryptococcus neoformans. It is a soil saprophyte and is particularly abundant in the feces of pigeons( pigeon's dropping). It does not appear to infect birds, probably because of their high body temperature and infection throughout the world. Classification of Cryptococcus Kingdom: Fung Phylum: Basidiomycota Class: Tremellomycetes Order: Tremellales Family: Tremellaceae Genus: Cryptococcus Species: C. neoformans Other species are- C. albidus ( nitrate...