universe84a

Klebsiella pneumoniae on MacConkey Agar

Klebsiella pneumoniae on MacConkey agar is a mucoid and lactose fermenter isolated from urine as shown above picture.

K. pneumoniae scientific classification is as follows-

• Domain: Bacteria
• Phylum: Proteobacteria
• Class: Gammaproteobacteria
• Order: Enterobacterales
• Family: Enterobacteriaceae
• Genus: Klebsiella
• Species:  pneumoniae
• Subspcecies of Klebsiella pneumoniae are-
• Klebsiella pneumoniae subspecies ozaenae
• Klebsiella pneumoniae subspecies  pneumoniae
• Klebsiella pneumoniae subspecies rhinoscleromatis

Klebsiella is from the surname of  German-Swiss microbiologist Edwin Klebs (1834–1913). Klebsiella is a genus of Gram-negative, oxidase-negative, fimbriated, non -motile, non-sporing rod-shaped bacteria with a prominent polysaccharide-based capsule and having size o,f   0.5 -0.8 µm wide to 1 -2  µm. They are found everywhere in nature and also in bacterial flora in our intestines.

Pathogenicity of Klebsiella pneumoniae

It may cause severe bronchopneumonia, urinary tract infections, nosocomial infections, wound infections, septicemia, meningitis, and rarely diarrhea too.

Isolation  in laboratory 

Klebsiella pneumoniae isolated from septicemic patient blood culture; on the basis of the following tests:-

Blood culture bottle incubated into Bactec.

After 23 hours of incubation, Bactec indicated positive.

The positive bottle was then sub-cultured into solid media Chocolate agar, blood agar, and MacConkey agar.

After overnight incubation, growth was seen in all three plates.

MacConkey’s Agar

On MacConkey agar, the colonies appeared large, mucoid, and pink in color. The mucoid nature of colonies is due to capsular material produced by the organisms.

Gram stain

Short thick Gram-negative rods

Motility by hanging drop preparation method

Non-motile

Biochemical tests

Catalase test -Positive

Oxidase -Negative

Nitrate reduction test- Reduced to nitrite

Triple Sugar Iron  (TSI) agar–

Acid/acid

No hydrogen sulfide (H2S) production

Production of gas

Motility Indole Urease (MIU) test

Non-motile because of only strict growth around the stab line

Urea hydrolyzation test-Test should be positive but here found negative may be due to Klebsiella pneumoniae subspecies rhinoscleromatis.

Indole -Negative

Note: Klebsiella oxytoca is indole positive.

Citrate Utilization Test

Positive

Fermentation of sugars (glucose, lactose, and mannitol )- acid and gas

Methyl red test-Negative

Voges-Proskauer test-Positive

Lysine decarboxylase test-Positive

Antibiotic sensitivity test

Antibiotic sensitivity test is also suggestive for Klebsiella pneumoniae because of ampicillin resistance which is intrinsically resistant.

Treatment 

Clinical isolates of Klebsiella pneumoniae are resistant to a wide range of antimicrobial agents like ampicillin, amoxicillin, and carbenicillin. This resistance is due to the R plasmid present in these organisms. Organisms are usually sensitive to cephalosporins, trimethoprim, nitrofurantoin, amoxicillin-clavulanic acid, and aminoglycosides. However, plasmid-mediated resistance to gentamycin and various cephalosporins has been reported in hospital strains.

#Related Videos

Klebsiella pneumoniae -introduction, pathogenesis, lab diagnosis and treatment

#K. pneumoniae growth on NA, BAP & MAC and its biochemical test-

#K. pneumoniae glucose utilization test using Andrade’s indicator-

# K. pneumoniae  under Microscope ||K. pneumoniae  Gram Stain

#K. pneumoniae  growth on MacConkey agar, blood agar, and chocolate agar-

#Staphylococcus aureus , E. coli, and K. penumoniae on growth on various media

Further Readings

1. Bailey & Scott’s Diagnostic Microbiology. Editors: Bettey A. Forbes, Daniel F. Sahm & Alice S. Weissfeld, 12th ed 2007, Publisher Elsevier.
2. Clinical Microbiology Procedure Handbook Vol. I & II, Chief in editor H.D. Isenberg, Albert Einstein College of Medicine, New York, Publisher ASM (American Society for Microbiology), Washington DC.
3. Colour Atlas and Textbook of Diagnostic Microbiology. Editors: Koneman E.W., Allen D.D., Dowell V.R. Jr, and Sommers H.M.
4. Cowan & Steel’s Manual for identification of Medical Bacteria. Editors: G.I. Barron & R.K. Felthani, 3rd ed 1993, Publisher Cambridge University Press.
5. Jawetz, Melnick and Adelberg’s Medical Microbiology. Editors: Geo. F. Brook, Janet S. Butel & Stephen A. Morse, 21st ed 1998, Publisher Appleton & Lance, Co Stamford Connecticut.
6. Mackie and Mc Cartney Practical Medical Microbiology. Editors: J.G. Colle, A.G. Fraser, B.P. Marmion, A. Simmous, 4th ed, Publisher Churchill Living Stone, New York, Melborne, Sans Franscisco 1996.
7.  Manual of Clinical Microbiology. Editors: P.R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover and R. H. Yolken, 7th ed 2005, Publisher ASM, USA
8.  Textbook of Diagnostic Microbiology. Editors: Connie R. Mahon, Donald G. Lehman & George Manuselis, 3rd edition2007, Publisher Elsevier.
9. Topley & Wilsons Principle of Bacteriology Vol I, Editors: M.T. Parker & L.H. Collier, 8th ed 1990, Publisher Edward Arnold publication, London.
10. District Laboratory Practice in  Tropical Countries  –  Part-2-   Monica Cheesebrough-   2nd Edn Update