Enterobacteriaceae: Introduction, Phenotypic Features and Related Videos

E. coli on MacConkey agar enterobacteriaceae

  Enterobacteriaceae

The family Enterobacteriaceae is a very large group of gram negative bacteria that posses a number of common morphological and biochemical properties and similar DNA base compositions.

The widely used systems for the classification of enterobacteriaceae are

  1. Bergey’s manual
  2. Kauffmann and
  3. Edward -Ewing.

Even having certain differences, the general approach is same.

The  family is  a group of subdivision or tribes and  each tribe consists of one or more genera.Similarly, each genus consists of one or more sub genera and species. The species are classified into types like biotypes, serotypes, bacteriophage types and colicin types.

This family has following tribes

Tribe 1: Escherichiae

Genus 

  1. Escherichia 
  2. Edwardsiella
  3. Citrobacter
  4. Salmonella
  5. Shigella

Tribe 2: Klebsiellae

Genus

  1. Klebsiella
  2. Enterobacter
  3. Hafnia
  4. Serratia

Tribe 3: Proteeae

Genus

  1. Proteus
  2. Morgenella
  3. Providencia

Tribe 4: Erwinieae

Genus

  1. Erwinia

Phenotypic features

The family enterobacteriaceae has following general phenotypic  features-

  1. They are small gram negative rod.
  2. Having usual cell structure (non sporulated)
  3.  Motile by peritrichous flagella.
  4. Grow on ordinary medium.
  5. Grow both aerobically and anaerobically.
  6. Grow without addition of sodium chloride or other supplements.
  7. Grow well on MacConkey agar.
  8.  Active biochemically.
  9. Ferments D-glucose and other sugar, often with gas production.
  10. Catalase test positive.
  11. Oxidase test negative.
  12. Reduce nitrate to nitrite
  13. Contain the enterobacterial  common antigen (ECA) and
  14. They have a 39-59% guanine plus cytosine (G+C) content of DNA.

 Key Notes

  1. All the members of family enterobacteriaceae are catalase  positive except Shigella dysentery type 1.
  2. All the members of family enterobacteriaceae are oxidase negative except Plesiomonas shigelloides which is oxidase test positive.
  3. All oxidase positive bacteria  are catalase test  positive except Kingella, Eikenella and Cardiobacterium those are catalase test negative.

E. coli  the member of Enterobacteriaceae on MacConkey agar, blood agar and Chocolate agar as shown below-

#E. coli under microscope|| Gram stain ||Gram Negative bacilli or Gram negative rods as shown below-

# Escherichia coli growth on nutrient agar, MacConkey agar and Blood agar and also biochemical tests-
Tripple sugar Iron(TSI) agar
Sulphide Indole Motility (SIM) test
Citrate Utilization Test
Urea hydrolization Test as shown below

# Klebsiella pneumoniae growth on nutrient agar, MacConkey agar and Blood agar and also biochemical tests-
Tripple sugar Iron(TSI) agar
Sulphide Indole Motility (SIM) test
Citrate Utilization Test
Urea hydrolization Test as shown below

# Swarming growth of Proteus on blood agar as shown below-

Salmonella Typhi isolated from Blood Culture-
Growth of Salmonella Typhi on nutrient agar, MacConkey agar and blood agar
Growth of Salmonella in blood culture bottle
Biochemical tests of Salmonella Typhi in
TSI
SIM
Citrate agar
Urea agar

References

  1. Cowan & Steel’s Manual for identification of Medical Bacteria. Editors: G.I. Barron & R.K. Felthani, 3rd ed 1993, Publisher Cambridge University press.
  2. Bailey & Scott’s Diagnostic Microbiology. Editors: Bettey A. Forbes, Daniel F. Sahm & Alice S. Weissfeld, 12th ed 2007, Publisher Elsevier.
  3. Clinical Microbiology Procedure Hand book, Chief in editor H.D. Isenberg, Albert Einstein College of Medicine, New York, Publisher ASM (American Society for Microbiology), Washington DC.
  4. Colour Atlas and Text book of Diagnostic Microbiology. Editors: Koneman E.W., Allen D.D., Dowell V.R. Jr and Sommers H.M.
  5. Jawetz, Melnick and Adelberg’s Medical Microbiology. Editors: Geo. F. Brook, Janet S. Butel & Stephen A. Morse, 21st ed 1998, Publisher Appleton & Lance, Co Stamford Connecticut.
  6. Mackie and Mc Cartney Practical Medical Microbiology. Editors: J.G. Colle, A.G. Fraser, B.P. Marmion, A. Simmous, 4th ed, Publisher Churchill Living Stone, New York, Melborne, Sans Franscisco 1996.
  7.  Text book of Diagnostic Microbiology. Editors: Connie R. Mahon, Donald G. Lehman & George Manuselis, 3rd edition2007, Publisher Elsevier.

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