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DNA Hydrolysis Test: Introduction, Principle, Test Requirements, Test Procedure, Result and Interpretation and Keynotes
Introduction of DNA Hydrolysis Test DNA Hydrolysis Test is useful for presumptive identification of Staphylococcus aureus which produces the enzyme deoxyribonuclease from other Staphylococci which do not produce DNase. This test is also positive in the following organisms like Aeromonas spp., Vibrio cholerae Stenotrophomonas maltophilia, Moraxella catarrhalis, and Serratia spp. except for Serratia fonticola. Other names of this test are the DNase test and deoxyribonuclease test. Principle of DNA Hydrolysis Test Deoxyribonucleases (DNases) are enzymes that hydrolyze deoxyribonucleic acid (DNA) and release free nucleotides and phosphate. The DNases produced by...
Deoxyribonuclease Test: Introduction, Principle, Test Requirements, Test Procedure, Result and Interpretation and Keynotes
Introduction of Deoxyribonuclease Test Deoxyribonuclease Test brief form is DNase test which is useful for presumptive identification of Staphylococcus aureus which produces the enzyme deoxyribonuclease from other Staphylococci which do not produce DNase. This test is also positive in the following organisms like Aeromonas spp., Vibrio cholerae Stenotrophomonas maltophilia, Moraxella catarrhalis, and Serratia spp. except for Serratia fonticola. Principle of Deoxyribonuclease Test Deoxyribonucleases (DNases) are enzymes that hydrolyze deoxyribonucleic acid (DNA) and release free nucleotides and phosphate. The DNases produced by bacteria are extracellular endonucleases that cleave DNA, yielding a...
Acetate Utilization Test: Introduction, Principle, Test Requirements, Test Procedure, Result and Interpretation and Keynotes
Introduction of Acetate Utilization Test Acetate Utilization Test is applied to determine the ability of an organism to use acetate as the sole source of carbon same as in the citrate utilization test for Enterobacteriaceae and it is recommended for the differentiation of Shigella species from Escherichia coli. Principle of Acetate Utilization Test Breakdown of the sodium acetate causes the pH of the medium to shift toward the alkaline range, turning the indicator, bromothymol blue from...