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Introduction of Chocolate Agar (CHOC)  CHOC stands for Chocolate agar and it is a non-selective, enriched growth medium that is the lysed blood agar. The agar is named for its color when the red blood cells (RBCs) lysis gives the medium a chocolate-brown color without having chocolate products. It is used for the isolation of fastidious bacteria, such as Haemophilus influenzae, when incubated at 35-37°C in a 5% CO2  incubator. Tool and techniques for isolation of Haemophilus influenzae as shown below-...

Introduction of MAC MAC stands for MacConkey medium or agar and it uses for the culture of gram-negative bacteria and therefore Enterobacteriaceae belonging bacteria grow well on this medium and coliforms also enjoy this medium. MacConkey Agar is a modification of Neutral Red Bile Salt Agar developed by MacConkey. It was one of the earliest culture media for the cultivation and identification of enteric organisms from clinical specimens as well as food and water. It is a selective, differential,...

Slit Skin Smear Staining Introduction Slit Skin Smear Staining is a very useful technique to see the presence of the causative organism, Mycobacterium leprae, in the skin smear of a patient’s body since slit skin smear (SSS) is a gold standard technique for the leprosy diagnosis. The causative agent of leprosy, also called Hansen' disease is M. leprae and three cardinal signs of leprosy are as follows- Hypo or hyperpigmented anaesthetic patch Peripheral nerve involvement Positive...