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Introduction of 4-Methylumbelliferyl-β-D Glucuronide Test 4-Methylumbelliferyl-β-D Glucuronide Test short form is MUG test and it acts as a substrate for the organisms those having the enzyme β-glucuronidase. This enzyme is present in 97% of strains of E. coli. Organisms other than E. coli of the Enterobacteriaceae family  Salmonella, Shigella, and  Yersinia also possess the enzyme β-glucuronidase. This test uses for rapid identification of E. coli, the most common gram-negative rod seen in clinical specimens. Since verotoxin-producing E. coli strains...

Introduction of Methyl-α-D-Glucopyranoside Test Methyl-α-D-Glucopyranoside Test short form is MGP test. MGP test is effective for species identification of Enterococcus. Enterococcus gallinarum and Enterococcus casseliflavus are MGP test positive while Enterococcus faecalis and Enterococcus faecium are negative. Vancomycin-resistant Enterococcus faecium (VREF) is a serious nosocomial problem that is difficult to differentiate from  Enterococcus gallinarum and Enterococcus casseliflavus, while not important for purposes of control of nosocomial infections, show intrinsic resistance to vancomycin due to the vanC gene....

Introduction of Cooked Meat Broth Cooked Meat Broth or Robertson’s Cooked Meat (RCM) Medium is the recommended medium for the cultivation of anaerobic as well as aerobic bacteria. It is especially for pathogenic Clostridium species from clinical, food, and water samples. This can also be used as a maintenance medium for stock cultures of anaerobes as well as aerobes. RCM is called cooked meat broth (CMB) since it contains pieces of fat-free minced cooked meat of ox...