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Urea hydrolyzation Test: Principle, Procedure, Interpretation, Urease Positive Organisms, Limitations and Keynotes
Introduction of Urea Test / Urea hydrolization Test / Urease Test Urea Test or Urea hydrolyzation Test or Urease Test are other names of the same test. The urease test is important for certain bacteria and fungi that possess this enzyme that hydrolyzes urea releasing ammonia into the medium. This produces a change in the pH of the medium that can be detected by the color change in the indicator dye. This test can be used to...
Tryptone Water: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes
Introduction of Tryptone Water Tryptone water is used for the detection of indole production by coliforms ( E. coli, Klebsiella oxytoca). Principle of Tryptone Water Water is the source of hydrogen and oxygen. Sodium chloride is the source of electrolytes. Casein enzymic hydrolysate is a good substrate for indole production due to its high tryptophan content. Certain bacteria break down the amino acid tryptophan with the help of enzymes that mediate the production of indole by...
SYBR Safe DNA Gel Stain: Introduction, Other SYBR Dyes, Sensitivity, Ease of Use, Compatibility and Flexibility
Introduction of SYBR Safe DNA Gel Stain SYBR Safe DNA Gel Stain name is a DNA stain but it also stains RNA. It is a highly sensitive stain for visualization of DNA in agarose or acrylamide gels. It is specifically formulated to be a less hazardous alternative to commonly using ethidium bromide that can utilize either blue light or UV excitation. It is beneficial for us for the following purposes- It reduces exposure to highly mutagenic...