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Slit Skin Smear Staining Introduction Slit Skin Smear Staining is a very useful technique to see the presence of the causative organism, Mycobacterium leprae, in the skin smear of a patient’s body since slit skin smear (SSS) is a gold standard technique for the leprosy diagnosis. The causative agent of leprosy, also called Hansen' disease is M. leprae and three cardinal signs of leprosy are as follows- Hypo or hyperpigmented anaesthetic patch Peripheral nerve involvement Positive...

Introduction of Germ Tube Test (GTT) GTT stands for Germ Tube Test and GTT procedure is used for presumptive identification of Candida species. The culture of Candida species is treated with normal human pooled serum or sheep or rabbit or fetal calf serum and incubated at 37°C for 2-4 hours. A drop of suspension is examined on the slide under a microscope. The germ tubes are seen as long as tube-like projections extending from the yeast cells. There...

Introduction of CLED Medium CLED stands for cysteine, lactose, and electrolyte-deficient. CLED  medium or agar is a differential culture medium used for isolation and enumeration of bacteria in urine specimens from suspected cases of urinary tract infection (UTI). It favors the growth of all potential urinary pathogens as well as a number of contaminants such as diphtheroid, lactobacilli, and micrococci. Candida also enjoys this medium. This is a sole medium alternate for routine urine culture, inoculation...