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Introduction of Medical Mycology Information links Nearly 1.5 million environmental fungal species out of which 8000 plant pathogens and 300 human pathogens respectively but such scenarios have changed rapidly in recent years. Prolonged steroid use, recent neutropenia, diabetes mellitus, cancer (AML), and rheumatologic conditions are the principal predisposing factors to fungal infections and they are increasing day by day. The responsibility for national/ international surveillance like fungal infection rate, antifungal resistance surveillance, and for outbreak investigation are...

Use of Biphasic Medium Biphasic medium or diphasic medium is called so because of containing medium both solid and liquid phases in a single bottle. It uses mainly for minimizing contamination and spread of infections. It applies to cultures of highly infectious agents like Brucella and Mycobacterium. Isolation of Brucella  It requires a selective medium: It contains bacitracin, nalidixic acid, cycloheximide, nystatin, polymyxin B, and vancomycin in a serum dextrose agar base. Bone marrow culture -criterion standard samples of any...

Introduction of Lowenstein-Jensen (LJ) Medium Lowenstein-Jensen (LJ) Medium is named after the surnames of  Austrian pathologist Ernst Lowenstein (1878–1950) and the Danish chemist Kai Arne Jensen (1908–1992). The causative agent of tuberculosis is Mycobacterium tuberculosis. M. tuberculosis requires aerobic conditions and a protein-enriched medium for culture. The brief name of Lowenstein–Jensen medium is LJ medium. LJ  medium slopes are the main solid media used to culture Mycobacterium species( especially Mycobacterium tuberculosis) that contains inspissated eggs, malachite green, and glycerol. LJ medium containing...