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SARS‑CoV‑2 RNA Extraction using Biocomma Kit: Introduction, Reagent Preparation, Extraction Requirements, Procedure, and Keynotes
Introduction of SARS‑CoV‑2 RNA Extraction using Biocomma Kit SARS‑CoV‑2 RNA Extraction or Biocomma nucleic acid (DNA/RNA) extraction kit is a Chinese product that is useful for RNA extraction systems for COVID-19 detection. It is applied for manual extraction and it is completed in four steps- Lysis Binding Washing and Elution. A kit (for 50 tests) contains- Lysis buffer(GLX)-30 ml Proteinase K-20 mg/ml (1 ml) Spin column and collection tube(50/50 pieces) Wash buffer-1(PD)- 15 ml+15 ml isopropanol...
Alkaptonuria: Introduction, Symptoms, Causes, Laboratory Diagnosis, Treatment, and Keynotes
Introduction of Alkaptonuria Alkaptonuria is an enzyme deficiency, homogentisic dioxygenase (HGD) rare inherited disorder. It is used to break down a toxic substance called homogentisic acid. Excess to homogentisic acid causes bones and cartilage to become discolored and brittle which causes later osteoarthritis, especially in the spine and large joints. The patient who has alkaptonuria also has urine that turns dark brown or black when it is exposed to air (as shown in the above image)....
Hepatitis A Virus (HAV) Rapid IgM and IgG Test: Introduction, Principle, Procedure, Result Interpretation, Limitations and Keynotes
Hepatitis A virus (HAV) Rapid IgM and IgG Test Hepatitis A virus or HAV rapid test is used for the detection of immunoglobulin G(IgG) and immunoglobulin M (IgM) of past and present infections respectively as shown above picture. Hepatitis A is an acute, usually self-limiting disease of the liver caused by HAV. It is transmitted from person to person, primarily by the feco-oral route. The incidence of hepatitis A is closely related to socioeconomic development, and seroepidemiological...