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PDA Test: Introduction, Principle, Test Requirements, Procedure, Result Interpretation, Keynotes and Limitations
PDA Test PDA stands for phenylalanine deaminase and PDA test positive and negative are as shown above image. It uses in the differentiation of gram-negative enteric bacilli based on the ability of the microorganisms to produce phenyl pyruvic acid by oxidative deamination. The deamination of phenylalanine by oxidative enzymes results in the formation of phenyl pyruvic acid. After incubation, an aqueous solution of ferric chloride is added. If phenyl pyruvic acid is present, a light to deep green...
HAV Rapid IgM and IgG Test: Introduction, Principle, Procedure, Result Interpretation, Limitations and Keynotes
Hepatitis A virus (HAV) Rapid IgM and IgG Test Hepatitis A virus or HAV rapid test is used for detection of immunoglobulin G(IgG) and immunoglobulin M (IgM) of past and present infection respectively as shown above picture. Hepatitis A is an acute, usually self-limiting disease of the liver caused by HAV. It is transmitted from person to person, primarily by the feco-oral route. The incidence of hepatitis A is closely related to socioeconomic development, and seroepidemiological studies...
TBA for Corynebacterium: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes
Introduction of Tellurite Blood Agar (TBA) TBA stands for Tellurite Blood Agar and it is applied for the selective isolation and cultivation of Corynebacterium species and they are Gram-positive, facultatively anaerobic, non-motile bacteria that exhibit a fermentative metabolism (carbohydrates to lactic acid) under certain conditions. Most species are normal flora of humans present virtually at all anatomic sites. Tellurite Blood Agar is a selective medium used for the isolation and cultivation of Corynebacterium species. It is...