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SS Agar: Introduction, Composition, Principle, Procedure, Results- Interpretation, Uses,Limitations, and Keynotes
Introduction of SS Agar SS Agar is the short form of Salmonella-Shigella Agar and it is recommended for use as a selective and differential medium for the isolation of Salmonella and some Shigella species from clinical and non-clinical specimens (suspected foodstuffs). Composition of SS Agar (Himedia) Ingredients Gms / Litre Proteose peptone 5.0 Lactose 10.0 Bile salts mixture 8.5 Sodium citrate 8.5 Sodium thiosulphate 8.5 Ferric citrate 1.0 Brilliant green 0.00033 Neutral red 0.025 Agar 13.5...
PPLO Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes
Introduction of PPLO Agar PPLO Agar is recommended for the isolation and cultivation of Mycoplasma species. Mycoplasma is also called Pleuropneumonia-like organisms. PPLO is the short form of this organism. This medium is for Mycoplasma and therefore it is called PPLO agar. It is also known as Mycoplasma agar. Principle of PPLO Agar The culture medium for the cultivation of Mycoplasma should be free of any toxic substances even in small amounts. Beef heart infusion from peptone...
Brilliant Green Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes
Introduction of Brilliant Green Agar Brilliant Green Agar is a selective medium for the isolation of Salmonellae other than Salmonella Typhi from feces, foods, and dairy products. Principle of Brilliant Green Agar The incorporation of peptone, tryptone, and yeast extract makes the medium highly nutritious. It supplies amino acids and long chains of peptides. Sodium chloride is responsible for the osmotic equilibrium. Lactose and sucrose both are fermentable carbohydrate sources. Phenol red is an acid-base indicator....