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Troponin Test: Introduction, Testing Conditions, Types, Clinical Significance, and Keynotes
Introduction of Troponin Test The high-sensitivity cardiac troponin test (hs-cTnT) is the new generation of heart/cardiac enzyme assaying that allows for the detection of very low levels of troponin T, assisting to diagnose heart attacks more quickly. It works on the principle of immunochromatography. It is a simple, rapid, and quick/spot test. If the test is negative, it can also hint “rule out” heart damage from coronary artery disease (CAD). Testing Conditions of hs-cTnT No fasting...
Urine Casts: Introduction, Identification Features, and Clinical Significance
Introductions of Common Urinary Casts Urinary or urine casts in urine routine examination are completed in the following steps- First request the patient to bring a urine specimen. After the arrival of the specimen in the clinical laboratory, take nearly 5 ml of urine and centrifuge it. Put a drop of the mixed sediment part on a clean and grease-free glass slide and place a coverslip over it. Examine the entire 22- by 22-mm coverslip systematically...
SARS‑CoV‑2 RNA Extraction using Biocomma Kit: Introduction, Reagent Preparation, Extraction Requirements, Procedure, and Keynotes
Introduction of SARS‑CoV‑2 RNA Extraction using Biocomma Kit SARS‑CoV‑2 RNA Extraction or Biocomma nucleic acid (DNA/RNA) extraction kit is a Chinese product that is useful for RNA extraction systems for COVID-19 detection. It is applied for manual extraction and it is completed in four steps- Lysis Binding Washing and Elution. A kit (for 50 tests) contains- Lysis buffer(GLX)-30 ml Proteinase K-20 mg/ml (1 ml) Spin column and collection tube(50/50 pieces) Wash buffer-1(PD)- 15 ml+15 ml isopropanol...