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PPLO Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes
Introduction of PPLO Agar PPLO Agar is recommended for the isolation and cultivation of Mycoplasma species. Mycoplasma is also called Pleuropneumonia-like organisms. PPLO is the short form of this organism. This medium is for Mycoplasma and therefore it is called PPLO agar. It is also known as Mycoplasma agar. Principle of PPLO Agar The culture medium for the cultivation of Mycoplasma should be free of any toxic substances even in small amounts. Beef heart infusion from peptone...
Brilliant Green Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes
Introduction of Brilliant Green Agar Brilliant Green Agar is a selective medium for the isolation of Salmonellae other than Salmonella Typhi from feces, foods, and dairy products. Principle of Brilliant Green Agar The incorporation of peptone, tryptone, and yeast extract makes the medium highly nutritious. It supplies amino acids and long chains of peptides. Sodium chloride is responsible for the osmotic equilibrium. Lactose and sucrose both are fermentable carbohydrate sources. Phenol red is an acid-base indicator....
Cetrimide Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes
Introduction of Cetrimide Agar Cetrimide agar is a selective medium used for the isolation of Pseudomonas aeruginosa. Principle of Cetrimide Agar Cetrimide of the medium inhibits bacteria other than Pseudomonas aeruginosa. It is a cationic detergent that acts as a quaternary ammonium compound, which causes nitrogen and phosphorus to be released from cells of bacteria other than P. aeruginosa . Magnesium chloride and potassium sulfate in the medium enhance the production of the pigment pyocyanin, which is a...