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Acetamide Utilization Test: Introduction, Composition, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes
Introduction of Acetamide Utilization Test Acetamide (ethanamide) is the simplest amide derived from acetic acid. The chemical formula of this organic compound is CH3CONH2. An acetamide utilization test is recommended for differentiating Pseudomonas aeruginosa from other non-glucose-fermenting, Gram-negative bacilli/rods. It differentiates bacteria based on utilizing acetamide as the sole carbon source. Composition of Acetamide Agar Ingredients Amount (gram/liter) Sodium chloride: 5.0 Magnesium sulfate: 0.2 Ammonium phosphate (NH4H2PO4), monobasic: 1.0 Potassium phosphate (K2HPO4), dibasic: 1.0 Acetamide: 10.0 Agar:...
Weil Felix Test for Rickettsial Diseases: Introduction, Principle, Procedure, Result Interpretation and Keynotes
Introduction of Weil Felix Test for Rickettsial Diseases Weil Felix test is a serological assay that is used for the diagnosis of rickettsial infection by indicating immunoglobulins/antibodies in the serum. In this test, OX -19, OX -2 strain of Proteus vulgaris, and OX-K of P. mirabilis are used instead of specific rickettsial pathogens as antigens. This test was developed from the observation that certain strains of Proteus species isolated from the patient's urine with epidemic typhus were...
Respiratory Syncytial Virus: Introduction, Pathogenicity, Lab Diagnosis, and Treatment
Introduction of Respiratory Syncytial Virus Respiratory syncytial virus (RSV) is a common respiratory virus that normally causes mild, cold-like symptoms. Most of the sufferers recover in a week or two, but RSV can be serious, particularly for infants and older adults. Some features of this virus are- belongs to the family Paramyxoviridae Enveloped RNA virus Pleomorphic with helical capsid symmetry 90-130 nm in size Transmission and pathogenicity of RSV Person to person transmission is mainly by...