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Dubos Oleic Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes
Introduction of Dubos Oleic Agar Dubos Oleic Agar is commonly used for isolation and antimicrobial susceptibility testing (AST) of Mycobacterium tuberculosis . M. tuberculosis, the etiological agent of tuberculosis. The organism may present in airborne particles(droplet nuclei)that are generated when patients with pulmonary tuberculosis cough. The disease can happen when a susceptible person inhales the droplet nuclei containing M. tuberculosis. It is one of the common agar-based media for the isolation of mycobacteria. Principle of Dubos Oleic Agar...
Agarose Gel Electrophoresis: Introduction, Principle, Requirements, Test Procedure, and Applications
Introduction of Agarose Gel Electrophoresis Agarose gel electrophoresis is a method of gel electrophoresis. It is widely applied in various fields like biochemistry, molecular biology, genetics, clinical chemistry, etc. to separate a mixed population of macromolecules such as DNA, RNA, or proteins in a matrix of agarose. Agarose is a natural linear polymer and it is extracted from seaweed that forms a gel matrix by hydrogen bonding when heated in a buffer and allowed to cool. They are the most popular solid...
Schaedler Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes
Introduction of Schaedler Agar Schaedler Agar is recommended for the enumeration of various aerobic and anaerobic bacteria present in the gastrointestinal tract. Bacteroides fragilis grew on Schaedler Agar after 48 hours of anaerobic incubation as shown above picture. Principle of Schaedler Agar Tryptone, proteose peptone, Soya peptone, Yeast extract, and L-cystine supply nitrogenous growth factors, vitamins, and other essential growth nutrients. Dextrose/glucose serves as an energy source. Hemin and sheep blood enhances the growth of fastidious microbes and...