Introduction of Fungal Culture Media Fungal Culture Media are very important for the isolation of fungal etiological agents from clinical specimens in the Clinical Mycology Laboratory and their detailed study. The common fungal culture media are Sabouraud Dextrose Agar (SDA), Potato Dextrose Agar (PDA), Corn Meal Agar (CMA), Dermatophyte Test Medium (DTM), Czapek Dox Agar, Ascospore Agar, and Bird Seed Agar. The introduction, Principle, Preparation, Test Procedure, Colony Morphology, Result-Interpretation, and Keynotes of those fungal culture media are described...
Introduction of Campylobacter Blood Agar (CVA) Campylobacter Blood Agar (CVA) is a selective medium used for the primary isolation of Campylobacter jejuni from the clinical specimen, feces/stool. The recognized etiological agents of the gastrointestinal tract (GIT) infections are Campylobacter jejuni and Campylobacter coli. Blaser et al used a selective medium containing four antibiotics i.e. amphotericin, vancomycin, polymyxin B, and trimethoprim, and became a success in the isolation of Campylobacter jejuni in 1978. Later it was further modified by Reller et...
Introduction of Virus Cultivation in the Cell lines Viruses are intercellular pathogens that are dependent on host cell machinery for their multiplication and growth. Nowadays monolayer cell cultures are mostly used in diagnostic and research work in viral diseases. Cell culture is the most widely used system for the cultivation of viruses. It is a more convenient method compared to other methods like egg inoculation and animal inoculation. Candling of an egg is done prior to specimen...