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Cold staining for Mycobacterium lepare Principle of Staining of Mycobacterium leprae Mycobacterium leprae  (that cause leprosy) are extremely difficult to stain by ordinary methods because of the lipid-containing cell walls. They bind carbol -fuchsin tightly and resist destaining with strong decolorizing agents such as alcohol and strong acids. Acid-fast-negative bacteria readily lose the stain when treated with an acid-alcohol solution. The cold stain method is used for the detection of M. leprae and Nocardia asteroides (weakly acid-fast). Carbol-...

Introduction of Morgenella Morgenella morganii is a member of the family Enterobacteriaceae which was earlier called Proteus morganii. This genus Morgenella has only one species, M. morganii. It can be found in human and animal tissues and sewage, soil, and water. Pathogenecity of Morgenella It cause rarely urinary tract infections (UTI),hospital-acquired infections and it has also been reported as causing diarrhea in infants. Laboratory Diagnosis  of Morgenella Gram stain: Gram-negative bacilli Culture: Non-lactose fermenting colonies on MacConkey agar. Blood agar is...

Introduction of TSI Test This single TSI test is useful for the following features- To know the carbohydrate utilization  ability of the  organisms Gas formation by the organisms Hydrogen sulfide production by the bacteria TSI stands for triple sugar iron and it is an agar medium recommended for use in the differentiation of Enterobacteriaceae by their ability to ferment glucose, lactose, and sucrose, and their ability to produce hydrogen sulfide. Principle of TSI Test Triple sugar...