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E-Test (Epsilometer Test): Introduction, Principle, Procedure, Result and Interpretation
Introduction of Epsilometer test (E-test) Epsilometer test is in brief E-test. It is an automated system for measuring the minimum inhibitory concentration (MIC) of the bacterial isolate. It is very simple to perform MIC of the bacterial isolate as compared to other techniques like broth and agar dilution methods which are technically cumbersome. An elliptical zone of growth inhibition is seen around the strip after incubation. The MIC is read from the scale at the intersection of...
Culture media: Introduction, Composition and Types
Introduction of culture media Media –Plural While medium is singular Culture media are required to grow the organisms from infected material to identify the causative agent and its basic constituents are- Water: source of hydrogen and oxygen Electrolyte: Sodium chloride or other electrolytes Peptone: It is a complex mixture of partially digested protein. It contains proteoses, amino acids, polypeptides, phosphates, minerals (K, Mg) and accessory growth factors like nicotinic acid and riboflavin. Meat extract: Available commercially...
Aeromonas: Introduction, Pathogenecity, Lab Diagnosis and Treatment
Introduction of Aeromonas Aeromoas: Greek; aer/aeros =air, gas; feminine gender noun monas = unit, monad Aeromonas=gas(-producing) monad Aeromonas was originally placed in the family Vibrionanceae (1965 by veron), which also included the genera Vibrio, Aeromonas & Plesiomomas. Phylogenetic investigations indicated that the genus Aeromonas is not closely related to vibrios. So, it is removed from the family Vibrionanceae and transfer to the new family Aeromanadaceae (1986 by Colwell, MacDonell, and De Ley ) based upon 5s &...