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Introduction of Culture of anaerobic bacteria Obligate anaerobes can not grow in the presence of oxygen i.e. air. These organisms die rapidly on exposure to air, therefore for maintaining anaerobiosis various methods have been devised for anaerobic culture. Principle for Culture of anaerobic bacteria Mechanism of anaerobiosis achieves by following methods- Displacement of oxygen Absorption of oxygen Combustion of oxygen or their combinations Deep nutrient agar tube is the simplest method. Anaerobes grow in the depth of the...

Indole test for bacteria This indole test is useful for both aerobic and anaerobic gram-negative rods and really very useful in the routine identification of bacteria in Microbiology. Principle of Indole test The ability of bacteria to split indole from the amino acid tryptophan is due to the presence of the enzyme, tryptophanase.  Indole,  if present combines with an aldehyde in the reagent to produce a pink to red-violet quinoidal compound if using benzaldehyde reagent or...

HBsAg Test Positive HBsAg test Positive as shown above picture. As you know, hepatitis B is the most common serious liver infection in the world. hepatitis B virus is responsible for this that attacks and injures the liver. According to the Hepatitis B Foundation, every year up to 1 million people die from hepatitis B despite the fact that it is preventable and treatable. The hepatitis B virus (HBV) transmits through blood and infected bodily fluids. It...