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Formal gel test for Visceral leishmaniasis: Introduction, principle, procedure and result interpretation
Introduction of formal gel test for visceral leishmaniasis Visceral leishmaniasis or Kala-Azar is a disease caused by Leishmania donovani. The disease has following features like remittent type of fever, massive enlargement of the spleen and generalized weakness. It is fatal if untreated within 2 years. Formal gel test is also called Napier's aldehyde test. It is a non specific test. Specimen for formal gel test for visceral leishmaniasis serum Method Chemical: Formal gel test Principle of formal gel...
Charcot Leyden crystal : Introduction, Detection and Its Clinical Significance
Introduction of Charcot Leyden crystal Charcot Leyden crystal is from surnames of describers Jean-Martin Charcot and Ernst Viktor von Leyden. It composes of an eosinophilic lysophospholipase binding protein called Galectin -10. It is a microscopic crystal and observable under microscope in specimens like stool, sputum and pleural fluid of people who are in allergic and parasitic diseases. Charcot–Leyden crystal appearance It varies in size and may be up to 50 µm. It is slender and pointed at both...
Culture of Anaerobic Bacteria: Introduction, Principle, Procedure and Result Interpretation
Introduction of Culture of anaerobic bacteria Obligate anaerobes can not grow in the presence of oxygen i.e. air. These organisms die rapidly on exposure to air, therefore for maintaining anaerobiosis various methods have been devised for anaerobic culture. Principle for Culture of anaerobic bacteria Mechanism of anaerobiosis achieves by following methods- Displacement of oxygen Absorption of oxygen Combustion of oxygen or their combinations Deep nutrient agar tube is the simplest method. Anaerobes grow in the depth of the...