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Bacterial Toxins: Introduction and Differences between Exotoxins and Endotoxins

Bacterial toxins Exotoxins and endotoxins of bacterial toxins Bacterial toxins are of two types and they are - Exotoxins and Endotoxins. 1. Exotoxins Exotoxins of bacteria toxins are heat-labile proteins that are secreted by certain species of bacteria. They diffuse readily into the surrounding medium. They are highly potent even in small amounts and constitute some of the most poisonous substances known. Botulinum toxin is the most poisonous, it has been estimated that 3 kg of botulinum toxin...

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Oxidative Fermentative (OF) Test: Introduction, Principle, Procedure ,Results Interpretation and Limitations

Oxidative-Fermentative (OF) Test determines if certain gram-negative bacilli metabolize glucose by fermentation or aerobic respiration (oxidatively). During the anaerobic process of fermentation, pyruvate is converted to a variety of mixed acids depending on the type of fermentation. The high concentration of acid produced during fermentation will turn the indicator, bromthymol blue present in  OF  basal medium from green to yellow in the presence(oxidatively) or absence of oxygen (fermentatively) .Some nonfermenting gram-negative bacteria metabolize glucose using aerobic respiration and therefore only produce a small amount of weak acids during  glycolysis and Krebs cycle. The decrease amount of peptone and increase amount of glucose helps the detection of weak acids so produced. Dipotassium phosphate buffer present in the medium is useful to further promote acid detection. It is also useful to test  nonsacchrolytic (have no ability to use the carbohydrate in the media) activities of organisms. Introduction of  Oxidative Fermentative  Test OF stands for Oxidative Fermentative test. The OF basal media was developed by Hugh and Leifson in 1953. OF Basal Medium is recommended for the detection of oxidation or fermentation of carbohydrates by bacteria. This basal medium is useful to aid in the identification of gram-negative bacteria on the basis of their ability to oxidize or ferment a specific carbohydrate. As compared to other OF Media, Hugh and Leifson's formula employs a...

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Staphylococcus aureus: Introduction, Pathogenecity, Lab Diagnosis and Treatment

Staphylococcus aureus on blood agar Introduction of Staphylococcus aureus Scientific classification of Staphylococcus aureus is given as: Domain: Bacteria Phylum: Firmicutes Class: Bacilli Order: Bacillales Family: Staphylococceae Genus: Staphylococcus Species: aureus Staphyle means bunch and kokkos mean berry. It was isolated by Pasteur (1880) from a pus sample. The pathogenic role of Staphylococcus was described by Sir Alexander Ogston and who was a surgeon from Scotland. Other than Staphylococcus cluster forming cocci are- Micrococcus Gafkey Sarcina Peptococcus Aerococcus Staphylococcus aureus Definition...

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