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Deoxycholate Citrate Agar (DCA): Introduction, Principle, Composition, Test Procedure, Colony Characteristics and Uses

Deoxycholate Citrate Agar (DCA) is a  recommended medium for the isolation of enteric pathogens particularly Salmonella and Shigella species from clinical  as well food samples. DCA is a modification of Desoxycholate Agar formulated by Leifson. His original medium demonstrated improved recovery of intestinal pathogens from specimens containing normal intestinal flora by using citrates and sodium desoxycholate in specified amounts as inhibitors to gram-positive bacteria. Leifson modified his original medium by increasing the concentration of sodium citrate and sodium desoxycholate and found Desoxycholate Citrate Agar reliable for isolating many Salmonella and Shigella species. DCA  effectively isolates intestinal pathogens (Salmonella and Shigella species) by inhibiting coliforms and many Proteus species. Introduction of Deoxycholate Citrate Agar (DCA) Deoxycholate Citrate Agar (DCA) is a  recommended medium for the isolation of enteric pathogens particularly Salmonella and Shigella species from clinical as well food samples. DCA is a modification of Desoxycholate Agar formulated by Leifson. His original medium demonstrated improved recovery of intestinal pathogens from specimens containing normal intestinal flora by using citrates and sodium desoxycholate in specified amounts as inhibitors to gram-positive bacteria. Leifson modified his original medium by increasing...

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Vaginal Wet Mount: Introduction, Principle, Preparation, Result Interpretation, Limitations and Related Videos

Vaginal Wet Mount: Introduction, Principle, Preparation, Result Interpretation, Limitations and Related Videos Introduction of Vaginal Wet Mount The vaginal wet mount also called the vaginitis test is very useful for diagnosis vaginal infections that could be causing vaginitis. Vaginitis encompasses a variety of disorders that cause infection or inflammation of the vagina. The causative agents of vaginitis can include bacteria, yeast, viruses, and parasites. Non-infectious vaginitis may also occur due to estrogen imbalance (low level)  that causes vaginal dryness as well as a reaction to vaginal products such as...

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Microbiology Practical Examination: Introduction, Requirements and Preparation

Introduction of Microbiology Practical Examination As you know, practical builds up your confidence level and therefore it is the integral part of your course of study. The Microbiology Practical Examination will cover the topics from all the parts of microbiology like bacteriology, parasitology, virology, mycology and immunology. The contents of this examination are given below as requirements. It includes viva voce ( oral examination) and spots or objective structured practical examinations (OSPE). Introduction of Microbiology Practical Examination As you know, practical builds up your confidence level and therefore it is an integral part of your course of study. The Microbiology Practical Examination will cover the topics from all the parts of microbiology like bacteriology, parasitology, virology, mycology, and immunology. The contents of this examination are given below as requirements. It includes viva voce ( oral examination) and spots or objective structured practical examinations (OSPE). Requirements for Microbiology Practical Examination Chocolate agar blood...

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