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Introduction of PLET Agar PLET agar is the selective medium for the isolation and cultivation of Bacillus anthracis. Principle of PLET Agar PLET Agar Base is the Knisley formulation. It is the best selective medium for the cultivation of Bacillus anthracis. Components of the medium like Beef heart infusion from solids and tryptose provide the carbonaceous and nitrogenous compounds necessary for growth whereas sodium chloride (NaCl) provides the osmotic equilibrium. Thallous acetate, polymyxin, lysozyme (FD185) are inhibitory agents...

Introduction of Bird Seed Agar Bird Seed Agar sounds seed of a bird but actually, it is a niger seed, botanical name Guizotia abyssinica. Niger seed contains caffeic acid that serves as a substrate for the detection of phenoloxidase, an enzyme produced by Cryptococcus neoformans. The action of phenoloxidase on caffeic acid results in the production of melanin which is occupied by the yeast cell wall forming a tan to reddish-brown pigmentation. Bird seed agar in brief is...

Introduction of Schaeffer–Fulton Stain for Spore Staining When conditions for vegetative growth are not favorable, especially when carbon and nitrogen become unavailable spore formation occurs Spore-forming species are represented in most classes, including the Bacilli, the Clostridia, the Erysipelotrichi are able to survive by forming resistant endospores. Spore formation involves a change in enzyme activity and morphology. The spore may be positioned at the end (terminal) of the bacterium or centrally (median). It may be round,...