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Introduction of Sulfide, Indole, Motility Test Sulfide, Indole, Motility brief form is  SIM  and the SIM medium is useful for the differentiation of gram-negative enteric bacilli. Sulfide, Indole, Motility Test helps to isolate the organisms on the basis of sulfide production, indole formation, and motility. Principle of Sulfide, Indole, Motility Test The medium having the constituents ferrous ammonium sulfate and sodium thiosulfate, which together serve as indicators for the production of hydrogen sulfide (H2S). Hydrogen sulfide...

Introduction of Ziehl-Neelsen Stain This acid-fast bacilli in brief AFB staining or Ziehl-Neelsen staining method is a modification of Ehrlich's (1882) method. Ziehl-Neelsen Stain name is from the surnames of German doctors, bacteriologist Franz Ziehl(1859-1926), and pathologist Friedrich Neelsen (1854-1898). Principle of Ziehl-Neelsen Stain The presence of higher alcohol, glycerol, fatty acid, and especially mycolic acid in the cell wall has been found responsible for keeping the acid-fast property of bacteria. Therefore, AFB staining is useful...

Microbiological Approaches for Early Diagnosis of Infection Microbiological approaches are very useful for the early diagnosis of infection since automation in clinical microbiology is progressing rapidly. Much attention has been given to molecular biological techniques for timely diagnosis of bacterial infection so that an appropriate therapy could be given to the patient if the nature of the infection is known as in the early stage of the disease. In this way, we can definitely reduce morbidity...