universe84a

Introduction of Mannitol Salt Agar Mannitol Salt Agar (MSA) is a selective, differential, and indicator medium that is used to isolate and identify S. aureus from the clinical specimens. Other Staphylococcus species and Micrococcus also grow on this medium. Selective due to is very high salt (7.5%) compared with other media Gram Positive Staphylococcus: not fermenting mannitol, the medium does not change color (e.g.  epidermidis whereas Staphylococcus aureus yellow color colonies) Gram-Positive Streptococcus: No growth and also Gram-Negative: No growth Differential due to mannitol (sugar)fermentation (whenever sugar is fermented acid is produced)...

Introduction of Bacterial Inoculation Method The bacterial inoculation method is necessary to inoculate the bacteria in the suitable media to study the characteristics of bacteria like isolation of pure culture from mixed, colony morphology, biochemical reactions, serotyping, antimicrobial sensitivity testing, etc.  Different inoculation methods used in bacteriology are the streak plating technique, spread plate technique, and agar stab technique. Types of Bacterial Inoculation Method Inoculation by streaking Inoculation by spreading Inoculation by stabbing Streak Plating Technique...

Introduction of Methylene Blue Stain Methylene Blue Stain, also called methylene blue aqueous is a simple stain and is used for capsule stain as well as simple staining for a quick and easy way to determine cell shape, size, and arrangements of bacteria. It is also applicable to differentiate bacteria from yeast cells and presumptively identify the bacterial isolate by a simple wet mount technique. Principle of Methylene Blue Stain The capsule is different from the slime layer that...