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Molecular Laboratory Build Up: Introduction, Design, Requirements, Laboratory Practices and Its Uses
Introduction of Molecular Laboratory Build Up Molecular Laboratory Build Up is important since the molecular laboratory has become a growing part of the clinical laboratory. It includes all tests and methods to identify a disease and understand the predisposition for a disease analyzing nucleic acid i.e. DNA or RNA of an organism. Rapid advances in molecular diagnostics enable basic research and result in practical diagnostic tests which reflects the importance of molecular laboratory. The basic application is to determine changes...
Loeffler Methylene Blue Stain: Introduction, Principle, Composition, Preparation, Staining Procedure, Result Interpretation and Keynotes
Introduction of Loeffler Methylene Blue Stain Loeffler methylene blue stain is a metachromatic stain and thus it is preferred for metachromatic staining especially for Corynebacterium diphtheriae and also as a counterstain in Acid-fast staining. Principle of Loeffler Methylene Blue Stain Loeffler Methylene Blue is used in the presumptive identification of the etiological agent of diphtheria, Corynebacterium diphtheriae. Metachromasia is a phenomenon or the property of certain biological materials of staining a different color from that of the...
BSA: Introduction, Principle, Composition, Test Procedure, Colony Characteristics and Uses
Introduction of BSA BSA stands for Bird Seed Agar which sounds seed of a bird but actually, it is a niger seed, botanical name Guizotia abyssinica. Niger seed contains caffeic acid that serves as a substrate for the detection of phenoloxidase, an enzyme produced by Cryptococcus neoformans. The action of phenoloxidase on caffeic acid results in the production of melanin which is occupied by the yeast cell wall forming a tan to reddish-brown pigmentation. Bird seed agar in brief...