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Chocolate Agar (CHOC): Introduction, Principle, Composition,Preparation, Colony Characteristics and Uses
Introduction of Chocolate Agar (CHOC) Chocolate Agar (CHOC) is a non-selective, enriched growth medium that is the lysed blood agar. The agar is named for its color when the red blood cells (RBCs) lysis gives the medium a chocolate-brown color without having chocolate products. It is used for the isolation of fastidious bacteria, such as Haemophilus influenzae, when incubated at 35-37°C in a 5% CO2 incubator. Tool and techniques for isolation of Haemophilus influenzae as shown below- Principle of Chocolate Agar...
MacConkey Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes
Introduction of MacConkey Agar MacConkey agar(MAC) uses for the culture of gram-negative bacteria and therefore Enterobacteriaceae belonging bacteria grow well on this medium and coliforms also enjoy this medium. MacConkey Agar is a modification of Neutral Red Bile Salt Agar developed by MacConkey. It was one of the earliest culture media for the cultivation and identification of enteric organisms from clinical specimens as well as food and water. It is a selective, differential, and indicator medium because of the...
Blood Agar (BA): Introduction, Composition, Preparation, Principle and Interpretation, Uses , Types of Hemolysis and Keynotes
Introduction of Blood Agar (BA) Blood agar (BA) is an enriched medium for bacteria. Fastidious organisms, such as streptococci, do not grow well on ordinary growth media. It is a type of growth medium i.e. trypticase soy agar enriched with 5% sheep blood or blood agar base with 5-10 % sterile sheep blood that encourages the growth of bacteria and their hemolysis, such as streptococci, that otherwise wouldn’t grow. Composition of Sheep Blood Agar Base Ingredients Gms / Litre Casein enzymic hydrolysate:14.0...