K pneumoniae: Introduction, Classification, Pathogenicity, Laboratory Diagnosis and Treatment
Introduction of K pneumoniae
Klebsiella is from the surname of German-Swiss microbiologist Edwin Klebs (1834–1913). Klebsiella is a genus of Gram-negative, oxidase-negative, fimbriated, non -motile, non-sporing rod-shaped bacteria with a prominent polysaccharide-based capsule and having size o,f 0.5 -0.8 µm wide to 1 -2 µm. They are found everywhere in nature and also in bacterial flora in our intestines. K pneumoniae scientific classification is as follows
The antibiotic sensitivity test was also suggestive for Klebsiella pneumoniae because of ampicillin resistance which is intrinsically resistant.
The following antibiotics are used for antibiotics sensitivity testing-
Nitrofurantoin ( in case of pf urine only)
Polymyxin B (E-strip)
Treatment of K pneumoniae
Clinical isolates of Klebsiella pneumoniae are resistant to a wide range of antimicrobial agents like ampicillin, Amoxycillin, and carbenicillin. This resistance is due to the R plasmid present in these organisms. Organisms are usually sensitive to cephalosporins, trimethoprim, nitrofurantoin, amoxicillin-clavulanic acid, and aminoglycosides. However, plasmid-mediated resistance to gentamycin and various cephalosporins has been reported in hospital strains.
Klebsiella pneumoniae Mucoid lactose fermenter colonies on Macconkey agar and Biochemical Tests-
Another beautiful mucoid colony morphology of K pneumoniae is shown below video clip-
Bailey & Scott’s Diagnostic Microbiology. Editors: Bettey A. Forbes, Daniel F. Sahm & Alice S. Weissfeld, 12th ed 2007, Publisher Elsevier.
Clinical Microbiology Procedure Handbook Vol. I & II, Chief in editor H.D. Isenberg, Albert Einstein College of Medicine, New York, Publisher ASM (American Society for Microbiology), Washington DC.
Colour Atlas and Textbook of Diagnostic Microbiology. Editors: Koneman E.W., Allen D.D., Dowell V.R. Jr, and Sommers H.M.
Cowan & Steel’s Manual for identification of Medical Bacteria. Editors: G.I. Barron & R.K. Felthani, 3rd ed 1993, Publisher Cambridge University Press.
Jawetz, Melnick and Adelberg’s Medical Microbiology. Editors: Geo. F. Brook, Janet S. Butel & Stephen A. Morse, 21st ed 1998, Publisher Appleton & Lance, Co Stamford Connecticut.
Mackie and Mc Cartney Practical Medical Microbiology. Editors: J.G. Colle, A.G. Fraser, B.P. Marmion, A. Simmous, 4th ed, Publisher Churchill Living Stone, New York, Melborne, Sans Franscisco 1996.
Manual of Clinical Microbiology. Editors: P.R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover and R. H. Yolken, 7th ed 2005, Publisher ASM, USA
Textbook of Diagnostic Microbiology. Editors: Connie R. Mahon, Donald G. Lehman & George Manuselis, 3rd edition2007, Publisher Elsevier.
Topley & Wilsons Principle of Bacteriology Vol I, Editors: M.T. Parker & L.H. Collier, 8th ed 1990, Publisher Edward Arnold publication, London.
District Laboratory Practice in Tropical Countries – Part-2- Monica Cheesebrough- 2nd Edn Update