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Oxidative-Fermentative (OF) Test determines if certain gram-negative bacilli metabolize glucose by fermentation or aerobic respiration (oxidatively). During the anaerobic process of fermentation, pyruvate is converted to a variety of mixed acids depending on the type of fermentation. The high concentration of acid produced during fermentation will turn the indicator, bromthymol blue present in OF basal medium from green to yellow in the presence(oxidatively) or absence of oxygen (fermentatively) .Some nonfermenting gram-negative bacteria metabolize glucose using aerobic respiration and therefore only produce a small amount of weak acids during glycolysis and Krebs cycle. The decrease amount of peptone and increase amount of glucose helps the detection of weak acids so produced. Dipotassium phosphate buffer present in the medium is useful to further promote acid detection. It is also useful to test nonsacchrolytic (have no ability to use the carbohydrate in the media) activities of organisms. — Oxidative Fermentative (OF) Test: Introduction, Principle, Procedure ,Results Interpretation and Limitations
Introduction of Oxidative Fermentative Test OF stands for Oxidative Fermentative test. The...

Diphtheria bacillus versus Diphtheroids — Diphtheria Bacillus Versus Diphtheroids: Introduction and Differences in Details
Introduction of Diphtheria bacillus versus Diphtheroids Diphtheria bacillus versus Diphtheroids can easily understand....

Streptococcus pneumoniae versus Viridans streptococci — Streptococcus pneumoniae Versus Viridans streptococci: Introduction and Differentiating Features
Introduction of Streptococcus pneumoniae versus Viridans streptococci Streptococcus pneumoniae versus Viridans...

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Bloodstream Infection(BSI): Introduction, List of common causative agents, and Keynotes