Category: medical

Potato Dextrose Agar (PDA): Introduction, Principle, Composition, Test Procedure, Colony Characteristics and Uses

Potato Dextrose Agar contains dehydrated Potato Infusion and Dextrose. Potato infusion provides a nutrient base for luxuriant growth of most fungi where as Dextrose serves as a growth stimulant. Agar in the medium acts the solidifying agent. PDA has further modified incorporating agents like tartaric acid, Chloramphenicol  and Chlortetracycline. The incorporation of tartaric acid (TA) in the medium lowers the pH to 3.5 which inhibits bacterial growth. Chloramphenicol acts as a selective agent to inhibit bacterial overgrowth of competing microorganisms from mixed specimens, while permitting the selective isolation of fungi. Application of Chlortetracycline in PDA is  for the evaluation of yeast and mold from cosmetic products. Introduction of Potato Dextrose Agar (PDA) Potato Dextrose Agar ( PDA)...

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SDA Fungal Medium: Introduction, Principle, Composition, Preparation, Colony Characteristics and Uses

SDA Fungal Medium: Introduction, Principle, Composition, Preparation, Colony Characteristics and Uses Introduction of SDA SDA stands for Sabouraud Dextrose Agar and  Sabouraud...

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LPCB Stain: Introduction, Principle, Procedure, Result Interpretation and Its Applications

LPCB stain stands for lactophenol cotton blue and it is a combination of  fixative , staining and clearing agent. LPCB uses both as a mounting fluid and a stain. This is used for staining and microscopic identification of fungi. Its contents functions are as follows Introduction of LPCB stain LPCB stain stands for lactophenol cotton...

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