Category: medical

Dermatophyte Test Medium (DTM): Introduction, Principle, Composition, Test Procedure, Colony Characteristics and Uses

Dermatophyte Test Medium (DTM) is a selective and differential medium recommended for the cultivation and isolation of pathogenic dermatophytic fungi. The Dermatophytes are a distinct group of fungi that infect the hair, skin and nails in humans  producing a variety of cutaneous infections known as ringworm. Dermatophytes is the group of three genera  like Trichophyton, Microsporum and Epidermatophyton are responsible for most of the cutaneous fungal infections. Introduction of Dermatophyte Test Medium (DTM) Dermatophyte Test Medium (DTM)...

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Czapek Dox Agar : Introduction, Principle, Composition, Test Procedure, Colony Characteristics and Uses

Czapek Dox Agar is a semisynthetic medium used for the cultivation of fungi, containing sodium nitrate as the sole source of nitrogen. Sucrose serves as the sole source of carbon while sodium nitrate serves as the sole source of nitrogen. Dipotassium phosphate buffers the medium. Magnesium sulphate, potassium chloride, ferrous sulphate serves as sources of essential ions. Agar acts as solidifying agent. In modified  Czapek Dox Agar,  magnesium glycerophosphate and potassium sulphate replace the magnesium sulphate and potassium phosphate of the original which prevents the precipitation of magnesium phosphate and it is also a highly satisfactory substrate for chlamydospore production by Candida albicans. Introduction of Czapek Dox Agar Czapek medium is also called...

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Corn Meal Agar (CMA): Introduction, Principle, Composition, Test Procedure, Colony Characteristics and Uses

Corn Meal Agar (CMA) is a well established fungal medium which is a suitable substrate for chlamydospore production by Candida albicans and the maintenance of fungal stock cultures.This is a very simple formulation containing only cornmeal infusion and agar. The addition of glucose (0.2 g% w/v) to CMA will enhance the chromogenesis of some species of Trichophyton e.g. Trichophyton rubrum. Corn Meal Agar is an enrichment medium developed by Hazen and Reed for use in the cultivation of fungi. Walker and Huppert, in 1960, found that the addition of Tween 80 to Corn Meal Agar resulted in rapid and abundant chlamydospore formation. Introduction of Corn Meal Agar (CMA) Corn Meal Agar (CMA)...

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