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Introduction of Optochin Sensitivity Test Optochin sensitivity test uses for presumptive identification of Streptococcus pneumoniae from viridans streptococci. Principle of Optochin Sensitivity Test Ethylhydrocupreine hydrochloride (Optochin), a quinine derivative, selectively inhibits the growth of S. pneumoniae at a very low concentration (5ug/ml). Optochin may also inhibit other viridians streptococci, but only at much higher concentrations. The test has a sensitivity of more than 95%. Optochin is water-soluble and diffuses readily into an agar medium. Therefore, filter paper impregnated...

Introduction of Bacitracin Sensitivity Test Bacitracin Sensitivity Test is applied to differentiate genera like Micrococcus and Stomatococcus from Staphylococcus when combined with other procedures such as the modified oxidase test.  It is useful to test for presumptive identification of Group A streptococci ( Streptococcus pyogenes)  Principle of Bacitracin Sensitivity Test Bacitracin is a polypeptide antibiotic derived from Bacillus subtilis that functions to block cell wall formation by interfering with the dephosphorylation of the lipid compound that carries peptidoglycans to...

Introduction of Hippurate Hydrolysis Test The Hippurate Hydrolysis Test is applied to determine the ability of the organism to produce hippuricase which hydrolyzes the substrate hippurate. It is useful in the identification of Streptococcus agalactiae, Campylobacter jejuni, Listeria monocytogenes, and Gardnerella vaginalis.  Principle of Hippurate Hydrolysis Test The end products of hydrolysis of the substrate hippurate by a constitutive enzyme hippuricase of the test organisms include glycine and benzoic acid.  Glycine is deaminated by the oxidizing agent,...