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TCBS Agar: Introduction, Composition, Uses of Ingredients, Test Procedure, Result Interpretation and Limitation
Introduction of TCBS Agar Vibrio cholerae is the causative agent of cholera and TCBS agar (Thiosulfate Citrate bile salts sucrose agar) a selective as well as differential culture medium used for selective isolation of this bacterium including its species from a variety of clinical and nonclinical specimens. TCBS agar has a very high pH (8.5-9.5) which suppresses the growth of intestinal flora other than Vibrio spp. The bile salts inhibit the growth of gram-positive microorganisms. Vibrio spp. produce either yellow or green colonies on TCBS agar,...
Culture Media pH Assay: Introduction, Requirements, Procedure , pH Correction and Keynotes
Introduction of Culture Media pH Assay Culture Media pH Assay is very useful during culture media preparation for providing favorable pH of the medium. ''pH'' indicates a number of ''H'' hydrogen ions concentrations. pH 7 indicates neutral pH. pH above 7 indicates alkaline pH. pH below 7 indicates acidic pH. Normally tap water is pH 6.8. Most of the pathogenic bacteria need a slightly alkaline pH of 6.2-7.4. Vibrio cholerae requires a more alkaline pH of 9.0....
Candle Jar: Introduction, Principle, Test Requirements, Test Procedure, and Keynotes
Introduction of Candle Jar Candle Jar is used to incubate the culture in an atmosphere containing 5- 7% CO2. It is the conventional technique alternative to a CO2 incubator and principally uses for capnophilic bacteria like Haemophilus influenzae, and Neisseria menigitidis. It is also helpful for Streptococcus pneumoniae. Requirement for Candle Jar in microbiology Candle jar Candle Lighter/ match Test organisms Culture plates Incubator Bunsen burner Inoculating loop Principle of Candle Jar A candle jar is a container into which...