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Air Microbiology: Introduction, Detection Methods, Procedure, Result Interpretation and Keynotes
Introduction of Air Microbiology Air microbiology is the study of microorganisms present in the atmospheric air that may be either bacteria, archaea, fungi, and viruses. It is a subarea of environmental microbiology. Here we discuss the presence of bacteria in the air. Demonstration estimation of the number of bacteria carrying particles in the air is significant and its bacteria load variation in the following situations. In the premises where safe working depends on the bacterial content...
Mannitol Salt Agar (MSA): Introduction, Composition, Preparation, Uses , Colony Characteristics and Keynotes
Introduction of Mannitol salt agar (MSA) Mannitol Salt Agar (MSA) is a selective, differential, and indicator medium which is used to isolate and identify Staphylococcus aureus from the clinical specimens. Other Staphylococcus species and Micrococcus also grow on this medium. Selective due to is very high salt (7.5%) compared with other media Gram-Positive Staphylococcus: not fermenting mannitol, the medium does not change color (e.g. epidermidis whereas Staphylococcus aureus yellow color colonies) Gram-Positive Streptococcus: No growth and also Gram-Negative: No growth Differential due to mannitol (sugar)fermentation (whenever sugar is fermented acid is produced) and...
KOH Test Positive Slide of Sputum: Introduction, Principle, Procedure, Result Interpretation and Uses
Introduction of KOH Wet Mount The KOH Wet Mount is very important for presumptive diagnosis of the type of fungal infections whether ringworm, aspergillosis, dermatomycosis, blastomycosis, mucormycosis, otomycosis, and so on. It is also helpful in the selection of appropriate culture media for the isolation of etiological fungal agents. Principle of KOH Wet Mount Preparation As you know, potassium hydroxide (KOH) is a strong alkali. When specimens such as pus, skin, hair, nails, or sputum mix...