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Use of Biphasic Medium Biphasic medium or diphasic medium is called so because of containing medium both solid and liquid phases in a single bottle. It uses mainly for minimizing contamination and spread of infections. It applies to cultures of highly infectious agents like Brucella and Mycobacterium. Isolation of Brucella  It requires a selective medium: It contains bacitracin, nalidixic acid, cycloheximide, nystatin, polymyxin B, and vancomycin in a serum dextrose agar base. Bone marrow culture -criterion standard samples of any...

Introduction of Lowenstein-Jensen (LJ) Medium Lowenstein-Jensen (LJ) Medium is named after the surnames of  Austrian pathologist Ernst Lowenstein (1878–1950) and the Danish chemist Kai Arne Jensen (1908–1992). The causative agent of tuberculosis is Mycobacterium tuberculosis. M. tuberculosis requires aerobic conditions and a protein-enriched medium for culture. The brief name of Lowenstein–Jensen medium is LJ medium. LJ  medium slopes are the main solid media used to culture Mycobacterium species( especially Mycobacterium tuberculosis) that contains inspissated eggs, malachite green, and glycerol. LJ medium containing...

Introduction of Dorset Egg Medium In 1903, Dorset inaugurated the first egg-based medium, and later it was further modified by  Löwenstein-Jensen and Ogawa using whole eggs as nutrients and malachite green to inhibit the growth of contaminants. Dorset Egg Medium is used for culturing mycobacteria and it is a non-selective medium well suited for the maintenance of pure cultures of Mycobacterium. The medium is also used for the maintenance and transport of Neisseria meningitidis and Haemophilus influenzae type b. Principle...