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HPV Genes detection using Real-Time PCR: Introduction, Principle, Test Requirements, Procedure, Result Interpretation and Keynotes

HPV Genes detection using Real-Time RT-PCR Introduction, Principle, Test Requirements, Procedure, Result Interpretation and Keynotes  Introduction of HPV Genes Detection Prior to dealing with HPV Genes Detection, let's discuss the virus. Human papillomaviruses (HPV) are small double-stranded (ds) DNA viruses that belong to the family of Papillomaviridae. having a circular genome of the size of approximately 7.9 kilobases. There are more than 100 types of HPV that have been identified, and so far among them.  Certain HPV types are called high-risk HPV (hrHPV) like HPV 16 and 18.  They are associated with the...

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Microbiology Reporting Techniques: Introduction, List of Templates, and Keynotes

Introduction of Microbiology Reporting Techniques Microbiology Reporting  Techniques are useful to provide similar report formats from different Microbiology Laboratory platforms that should sound Microbiologically clear and concise. It should also be compatible with International Guidelines of Clinical Microbiology Practice like the Clinical & Laboratory Standards Institute (CLSI) previous National Committee for Clinical Laboratory Standards (NCCLS) and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) Guidelines. Microbiology Reporting Techniques Templates Culture Negative Reports Urine Culture:  No growth after...

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Acetamide Utilization Test: Introduction, Composition, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes

Acetamide Utilization Test-Positive and Negative Bacteria Introduction of Acetamide Utilization Test Acetamide (ethanamide) is the simplest amide derived from acetic acid. The chemical formula of this organic compound is CH3CONH2. An acetamide utilization test is recommended for differentiating Pseudomonas aeruginosa from other non-glucose-fermenting, Gram-negative bacilli/rods. It differentiates bacteria based on utilizing acetamide as the sole carbon source. Composition of Acetamide Agar Ingredients   Amount  (gram/liter) Sodium chloride: 5.0 Magnesium sulfate: 0.2 Ammonium phosphate (NH4H2PO4), monobasic: 1.0 Potassium phosphate (K2HPO4), dibasic:  1.0 Acetamide:  10.0 Agar:...

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