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Bacitracin Test: Introduction, Principle, Procedure and Expected Results
Introduction of bacitracin Bacitracin is a peptide produced by Bacillus subtilis and was first isolated in 1945. It uses as an antibiotic and it disrupts Gram-positive bacteria by interfering with the cell wall and peptidoglycan synthesis. It is primarily used as a topical preparation because it can cause kidney damage when used internally. But here we discuss its diagnostic roles in microbiology. 0.04 U bacitracin uses for presumptive identification of Streptococcus pyogenes while 10 units for...
Catalase test : Introduction, Principle, Procedure , Result Interpretation and Precautions
Introduction of Catalase Test Catalase test is used to differentiate those bacteria that produce this enzyme, such as staphylococci, from non-catalase producing bacteria such as streptococci. Principle of Catalase Test Catalase acts as a catalyst in the breakdown of hydrogen peroxide into nascent oxygen and water. This nascent oxygen causes bubbling. An organism is tested for catalase production by bringing it into contact with hydrogen peroxide. Bubbles of oxygen are released if the organism is a...
Typhus: Introduction, types, Causes, Symptoms, Diagnosis and Treatment
Introduction of Typhus Typhus is a disease caused by bacteria called Rickettsia. Fleas, mites, lice, or ticks transmit it when they bite us. When arthropods carrying around Rickettsia bite, they transmit the bacteria that causes typhus. Scratching the bite further opens the skin and allows the bacteria greater access to the bloodstream. Once in the bloodstream, the bacteria continue to reproduce and grow. Types It is of three types endemic epidemic and scrub typhus: It is fully...