Tag: Principle of Maxam-Gilbert Method of Sequencing

DNA Sequencing: Introduction, Types, Principle, Procedure and Uses

DNA sequencing is a technique used to determine the precise order of the four nucleotide bases, adenine, guanine, cytosine and thymine which make up a strand of DNA. These bases provide the underlying genetic basis (the genotype) for telling a cell what to do, where to go and what kind of cell to become (the phenotype). Nucleotides are not the only determinants of phenotypes, but also they are essential to their formation. Each organism has a specific nucleotide base sequence. Sequencing an entire genome (all of an organism’s DNA) remains a complex job. It requires breaking the DNA of the genome into many smaller pieces, sequencing the pieces, and assembling the sequences into a single long "consensus." e.g. Sample Comparison of the DNA sequences of a nucleoprotein gene in infections of two patients with different strains of rabies are as follows- Introduction of DNA Sequencing DNA sequencing is a technique used...

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