Tag: Limitations of Acridine Orange Staining

Acridine Orange Staining: Introduction, Principle, Procedure, Result Interpretation and Uses

Acridine Orange Staining: Acridine orange is a fluorochrome stain and uses the rapid identification of Trichomonas vaginalis, yeast cells, and clue cells in vaginal smears. It can also use to detect intracellular gonococci, meningococci, and other bacteria particularly in blood cultures. It intercalates with the nucleic acid  i.e. either DNA or RNA present in organisms and fluoresce to emit various colors that help in differentiation. This binding is the result the electrostatic interactions of acridine molecule between the nucleic acid base pairs. It shows metachromatic properties, and thus is commonly used in fluorescence microscopy and flow cytometry analysis of cellular physiology and cell cycle status,  even including the fluorescent microscopic examination of microorganisms.  Introduction of  Acridine Orange Staining Acridine orange is a fluorochrome stain.  Acridine...

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