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Introduction of Mycology Video Collection

Mycology Video Collection is a genuine huge collections of fungal videos. It will not olny make easier mycology but also help to boost practical knowledge to identify fungal aetiological agents in the field of laboratory medicine. It is widely applicable in the section of medical laboratory like DMLT/CMLT, B.Sc. MLT, MMLT, M.Sc. Medical Micribiology and such related fields.

List of  Mycology Video Collection

1. Potassium hydroxide (KOH) mount
2. Gram stain of Yeast cells

3. Lactophenol cotton blue (LPCB) wet mount

4. Fungal growth on Sabouraud dextrose agar (SDA)

Mycology Video Collection with Description

• Fungal Hyphae in KOH mount of Pus discharge
• Septate hyphae with characteristic dichotomous branching  (at an angle of approximately 45°)-a typical characteristic of Aspergillus

• Yest cells in Grams stained smear of Candida culture Microscopy with various magnifications
• Yeast cells are Gram positive.
• Yeast cells of Candida albicans

• Aspergillus fumigatus growth on SDA and its LPCB Preparation showing conidia, conidiophores, phialides
• Aspergillus structures in LPCB mount-
• Conidia of Aspergillus
• Conidiophores of Aspergillus
• Phialides of Aspergillus
• Mycelium of Aspergillus
• Growth of Aspergillus fumigatus on SDA
• Aspergillus fumigatus isolated on the following basis- Colour of conidia arrangement of phialides

• Candida albicans growth on SDA
• Culture on Sabouraud Dextrose agar (SDA) at 37° C for 24-48 hours.
• Colony characteristics: Cream colored pasty and glistening

• Germ Tube Test / GTT Positive Candida albicans
• The test is carried out using 0.5 ml rabbit or human serum in which test yeast  cells are inoculated and incubated at 37°C for 2-3 hours. Put drop of this after 2-3 hours incubation on the slide and cover with the coverslip. Focus at 10X objective and finally observe at high power objective (40X) of compound microscope.
• Result InterpretationGerm tube test (GTT) positive:  Presence of sprouting yeast cellsGerm tube test negative: Absence of sprouting yeast cells

Mycology Video Collection

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