Medical Lab Technologist Post: Level, Examination Structure and Syllabus with Model Questions
Medical Lab Technologist Post
Level of Medical Lab Technologist Post: 7th and qualification are Bachelor in Medical Laboratory Technology (BMLT) or Bachelor of Science in Medical Laboratory Technology (B.Sc. MLT) or equivalent.
Examination Structure for Medical Lab Technologist Post
The examination will complete in two phases.
Phase first: Written Examination
Phase second: Group test and person Interview
The written examination will occur in two papers,
Paper 1: Multiple Choice Questions (MCQs)
Paper 2: Subjective
Syllabus for Medical Lab Technologist Post
Section A- 30 Marks
1. Hematology 30%
1.1. Cleaning of glassware and safety precaution in the laboratory
1.2. Collection and preservation of different samples for the laboratory
1.3. Preparation of chemicals and different stains for the hematological tests
1.4. Quality control in the laboratory
1.5. Formation and development of Erythrocytes, Leucocytes, thrombocytes
1.6. Principle and clinical procedure for
1.6.1 Hemoglobin estimation and its standard curve calibration
1.6.2 Total count of W.B.C., R.B.C., Platelets, and reticulocytes
1.6.3 E.S.R., B.T., C.T., and RBC indices
1.6.4 Foetal hemoglobin estimation
1.6.5 Coomb’s tests
1.6.6 Blood banking & Transfusion
1.6.7 Coagulation profile (mechanism, disorder & investigations)
1.6.8 LE cell preparation
1.6.9 Tissue parasite
1.6.10 Absolutes cell count
1.7 Characteristics of Anemia, Leukaemia, Polycythemia, Leukamoid reaction,
Thalassaemia & Haemoglobinopathies
1.8 Principles and procedure of Osmotic fragility tests and cytochemical stains
1.9 Principle and procedure of G6PD, Hemoglobin electrophoresis
1.10 Preparation of reagents for special hematological investigation
1.11 Waster Disposal and Total Quality Management
2. Section B- 30 Marks
Microbiology 30%
2.1 Bacteriology. 10%
2.1.1 classification of medically important bacteria
2.1.2 Characteristics of Microorganism: Prokaryotes, Eukaryotes, Viruses
2.1.3 Bacterial growth and nutritional requirements, uptake of nutrients,
growth phages and sporulation
2.1.4 Antimicrobial drugs and their mode of actions with reference to cell
wall, cell membrane, Nucleic acid, and protein synthesis
2.1.5 Different methods of sterilization and disinfection
2.1.6 Preparation of different media and ingredients uses and interpretation
2.1.7 Preparation of chemicals and stains
2.1.8 Cultural procedure of different samples aerobically and anaerobically
2.1.9 Identification of bacteria and confirmative tests serologically and
biochemically
2.1.10 Different staining methods of bacteria and their principles
2.1.11 TB Bacteriology and skin scraping for AFB
2.1.12 Water bacteriology
2.1.13 C.S.F. and cavity fluids for culture
2.2 Virology (Subsection 2.2& 2.3=10%)
2.2.1 Classification of medically important viruses and mode of infection
2.2.2 Characteristic of viruses, nature of viruses, viral structure and
replication
2.2.3 Definition of R.N.A. and D.N.A. viruses
2.2.4 Principle and methods of serological precede for HCV, HIV, HBsAg
and HEV etc
2.3 Parasitology
2.3.1 Classification of medically important
2.3.1.1 Protozoal parasites
2.3.1.2 Helminthic parasites
2.3.1.3 Blood parasites
2.3.1.4 Semen analysis
2.3.2 Methods of identification of different parasites from stool samples by
2.3.2.1 Wet preparation
2.3.2.2 Concentration methods
2.3.2.3 Cultural methods
2.3.3 Method of identification of blood parasites
2.3.4 Routine Examination and special test in Urine
2.4 Mycology (Subsection 2.4& 2.5=10%)
2.4.1 Identification of superficial, deep & systemic mycosis
2.4.2 Opportunistic mycosis
2.4.3 Examination and identification by different methods and culture
2.5 Immunology
2.5.1 Principle and procedure for the estimation of:
2.5.1.1 VDRL (RPR)
2.5.1.2 TPHA,
2.5.1.3 ASO
2.5.1.4 CRP
2.5.1.5 Rheumatoid factor
2.5.1.6 Pregnancy test
2.5.1.7 TORCH Range
2.5.1.8 Cancer Marker
2.5.1.9 Agglutination Reaction
2.5.1.10 Precipitation Reaction
2.5.1.11 Flocculation Reaction
2.5.1.12 ELISA
2.5.1.13 Haemagglutination Reaction
2.6 Waster Disposal and Total Quality Management
3. Section C- 20 Marks
3 Biochemistry 20%
3.1 Preparation of normal and molar solution
3.2 Preparation of different reagents required for biochemical test
3.3 Colorimeter and Spectro photometer
3.4 Flame Photometry
3.5 Carbohydrate metabolism:
3.5.1 Glycolysis
3.5.2 Glycogenesis
3.5.3 Glycogenolysis
3.5.4 Pentose phosphate pathway
3.5.5 Kreb’s cycle
3.5.6 Gluconeogenesis
3.6 Protein metabolism
3.6.1 Transamination
3.6.2 Deamination
3.6.3 Urea cycle
3.6.4 Nitrogen balance
3.6.5 Creatinine and creatinine formation
3.7 Lipid metabolism
3.7.1 ß-oxidation
3.7.2α-oxidation
3.7.3 -oxidation
3.7.4 Ketone bodies formation and their utilization
3.7.5 Ketosis
3.7.6 Cholesterol and triglycerides synthesis
3.8 Hormone
3.8.1 Introduction
3.8.2 Types
3.8.3 Origin
3.8.4 Definition
3.8.5 Classification
3.8.6 Regulation
3.8.7 Measurement by various methods including RIA, EIA
3.9 Principle and procedure of different methods for the estimation of biochemical tests
3.9.1 Sugar, Urea, Cratinine, Uric Acid, Billirubin, GPT, GOT, ALP, Lipid
profile, Cardiac profile, Renal function test, Liver Function Test,
Clearance study, Amylase, and Electrolytes
3.9.2 Cavity fluids examination
3.9.3 C.S.F. examination
3.9.4 24 hours Urine Protein
3.10 Waster Disposal and Total Quality Management
4. Section D- 20 Marks
4. Histology/cytology 20%
4.1 Preparation of different types of fixatives and their uses
4.2 Methods of decalcification
4.3 Methods of processing of tissues to prepare paraffin block tissue
4.4 Description of different types of microtome, their principles and methods of cutting section from the paraffin block tissue
4.5 Preparation of routine and special histological and cytological stains and
staining procedure
4.6 Principles and methods of staining and mounting the tissue section on the glass slides
4.7 Waster Disposal and Total Quality Management
Model Questions for Medical Lab Technologist Post
Tick on the appropriate answer
- All the following accelerates the erythrocyte sedimentation rate (ESR) except
A) Macrocytosis B) Microcytosis
C) Increased plasma Fibrinogen D) Increased plasma globulin
Correct Answer:- (B) - Which clotting factor’s deficiency is not associated with any clinically significant hemorrhagic disorder?
A) Factor II B) Factor VIII
C) Factor IX D) Factor XII
Correct Answer:- (D) - The reaction of surface antigen with insoluble particles of specific antibodies result in
A) Agglutination B) Flocculation
C) Complement fixation D) Precipitation
Correct Answer:- (A) - Which electrolyte level will be incorrect if a haemolysed serum sample is used for determination?
A) Sodium B) Potassium
C) Chloride D) Calcium
Correct Answer:- (B) - Acton is detected by a biochemical test called
A) Methyl red B) Voges proskaur
C) Oxidaase D) Indole
Correct Answer:- (B)
Sample questions for Medical Lab Technologist Post
- What are the lab investigations of hemolytic anemia?
- What do you understand about antibiotic sensitivity testing? Describe the Kirby Baer method of sensitivity testing.
- What do you understand by dimorphic fungi? Describe dermatophytes.
- What are the principles behind the ELISA test?
- How do you differentiate different types of Jaundice by laboratory investigations?