Acridine orange stained chromatoid bars of Entamoeba histolytica/ dispar

Acridine Orange Stained Chromatoid Bars of Entamoeba histolytica/ dispar: Introduction and Procedure

Acidine orange stained slide of chromatoid bars of E. histolytica or dispar

Chromatoid bar of E. histolytica

Fluorescence microscopy demonstrating acridine orange stained chromatoid bars of Entamoeba histolytica/ dispar as shown above image.

Introduction of Acridine orange

Acridine orange is a fluorochrome stain and uses the rapid identification of Trichomonas vaginalis, yeast cells, and clue cells in vaginal smears. It can also use to detect intracellular gonococci, meningococci, and other bacteria, particularly in blood cultures. Acridine orange is a fluorochrome that causes DNA to fluoresce green and RNA to fluoresce orange-red.

Procedure Of Acridine Orange Staining

Cover the unfixed dried smear with the acridine orange acid stain for 5–10 seconds and smear fixes due to being fixative is contained in the stain.
Now, wash off the stain, and decolorize the smear with alcohol saline solution for 5–10 seconds.
Rinse the smear with physiological saline, and place the slide in a draining rack.
Add a drop of saline or distilled water to the smear, and cover with a cover glass.

Observation

Examine first with the 10X objective to see the distribution of fluorescing material, and then with the 40X objective to identifyTrichomonas vaginalis and to detect yeast cells, bacteria, and parasites( chromatoid bars of Entamoeba histolytica/ dispar).

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