Bacterial Power: Introduction and TSI Test in Details

bacterial power

Bacterial Power

Bacterial power -Unity is the power shown by bacteria. The bacteria which are not visible by our naked eyes replacing the whole medium from the test tube as shown in the picture. So, imagine its power yourself. Organisms after overnight incubation at 37°C in triple sugar iron (TSI ) agar, so let’s know in detail about the TSI test.

Triple Sugar Iron Agar Test

Triple Sugar Iron Agar test is a combo test and useful for identification of mainly gram-negative bacteria, on the basis of the following tests-

  • fermenter or oxidizer or non-utilizer of carbohydrates like glucose, lactose, or sucrose
  • production of hydrogen sulfide ( H2S)
  • formation of gas

Procedure of Triple Sugar Iron Agar test

  1. Stab and streak the test organism on the surface of TSI.
  2. Incubate at 37ºC for 24 hours.
  3. Acid production limited only to the butt region of the tube is indicative glucose utilization, while acid production in slant and butt indicates sucrose or lactose fermentation.

Result and interpretation of Triple Sugar Iron Agar test

  1. Yellow (Acid)/ Yellow (Acid), Gas, H2S: Lactose/Sucrose fermenter, H2S producer.
  2. Red (Alkaline)/ Yellow (Acid), No gas, No  H2S: Only Glucose, not Lactose/Sucrose fermenter, anaerogenic, No H2S production.
  3. Red (Alkaline)/ No change: Glucose, Lactose, and Sucrose Non-fermenter.
  4. Yellow (Acid)/ No change: Glucose oxidizer
  5. No change/No change: Non-fermenter.

Further Readings

  1. Cowan & Steel’s Manual for identification of Medical Bacteria. Editors: G.I. Barron & R.K. Felthani, 3rd ed 1993, Publisher Cambridge University Press.
  2. Bailey & Scott’s Diagnostic Microbiology. Editors: Bettey A. Forbes, Daniel F. Sahm & Alice S. Weissfeld, 12th ed 2007, Publisher Elsevier.
  3. Clinical Microbiology Procedure Handbook, Chief in editor H.D. Isenberg, Albert Einstein College of Medicine, New York, Publisher ASM (American Society for Microbiology), Washington DC.
  4. Colour Atlas and Textbook of Diagnostic Microbiology. Editors: Koneman E.W., Allen D.D., Dowell V.R. Jr, and Sommers H.M.
  5. Jawetz, Melnick and Adelberg’s Medical Microbiology. Editors: Geo. F. Brook, Janet S. Butel & Stephen A. Morse, 21st ed 1998, Publisher Appleton & Lance, Co Stamford Connecticut.
  6. Mackie and Mc Cartney Practical Medical Microbiology. Editors: J.G. Colle, A.G. Fraser, B.P. Marmion, A. Simmous, 4th ed, Publisher Churchill Living Stone, New York, Melborne, Sans Franscisco 1996.
  7.  Textbook of Diagnostic Microbiology. Editors: Connie R. Mahon, Donald G. Lehman & George Manuselis, 3rd edition2007, Publisher Elsevier.
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