Category: medical

Corn Meal Agar (CMA): Introduction, Principle, Composition, Test Procedure, Colony Characteristics and Uses

Corn Meal Agar (CMA) is a well established fungal medium which is a suitable substrate for chlamydospore production by Candida albicans and the maintenance of fungal stock cultures.This is a very simple formulation containing only cornmeal infusion and agar. The addition of glucose (0.2 g% w/v) to CMA will enhance the chromogenesis of some species of Trichophyton e.g. Trichophyton rubrum. Corn Meal Agar is an enrichment medium developed by Hazen and Reed for use in the cultivation of fungi. Walker and Huppert, in 1960, found that the addition of Tween 80 to Corn Meal Agar resulted in rapid and abundant chlamydospore formation. Introduction of Corn Meal Agar (CMA) Corn Meal Agar (CMA)...

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Potato Dextrose Agar (PDA): Introduction, Principle, Composition, Test Procedure, Colony Characteristics and Uses

Potato Dextrose Agar contains dehydrated Potato Infusion and Dextrose. Potato infusion provides a nutrient base for luxuriant growth of most fungi where as Dextrose serves as a growth stimulant. Agar in the medium acts the solidifying agent. PDA has further modified incorporating agents like tartaric acid, Chloramphenicol  and Chlortetracycline. The incorporation of tartaric acid (TA) in the medium lowers the pH to 3.5 which inhibits bacterial growth. Chloramphenicol acts as a selective agent to inhibit bacterial overgrowth of competing microorganisms from mixed specimens, while permitting the selective isolation of fungi. Application of Chlortetracycline in PDA is  for the evaluation of yeast and mold from cosmetic products. Introduction of Potato Dextrose Agar (PDA) Potato Dextrose Agar ( PDA)...

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SDA Fungal Medium: Introduction, Principle, Composition, Preparation, Colony Characteristics and Uses

SDA Fungal Medium: Introduction, Principle, Composition, Preparation, Colony Characteristics and Uses Introduction of SDA SDA stands for Sabouraud Dextrose Agar and  Sabouraud...

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