Blog
Bile Esculin Test: Introduction,Principle, Test Requirements, Procedure, Result Interpretation and Limitations
Introduction of Bile Esculin test Bile esculin/aesculin test is widely used to differentiate Enterococci from streptococci, other than streptococcus bovis which are bile tolerant and can hydrolyze esculin to esculetin, it reacts with an iron salt in the medium to form a phenolic iron complex which produces a dark brown or black color. This test is based on the ability of Enterococcus species, to hydrolyze esculin in the presence of bile (4% bile salts or 40%...
Semen Test: Introduction, Physical, Chemical, Microscopic Examination, Normal Range of Sperm Count and Clinical Significance
Semen Test/ Analysis Semen Test or Semen analysis or a sperm count test is an indicator that shows the health and viability of a man’s sperm. Semen is the fluid containing sperm and it is released during ejaculation. A semen test evaluates the number, shape, and movement/ motility of the sperm. Generally, two or three separate sperm analyses are recommended to get a good idea of sperm condition. Semen Test Preparation Collection of samples: Recommended following...
BAP: Introduction, Composition, Preparation, Principle and Interpretation, Uses, Types of Hemolysis and Keynotes
Introduction of BAP BAP stands for 5% sheep blood agar plate and it is an enriched medium for bacteria. Fastidious organisms, such as streptococci, do not grow well on ordinary growth media. It is a type of growth medium i.e. trypticase soy agar enriched with 5% sheep blood or blood agar base with 5-10 % sterile sheep blood that encourages the growth of bacteria and their hemolysis, such as streptococci, that otherwise wouldn’t grow. Composition of Sheep Blood Agar Base Ingredients Gms / Litre Casein...